The bacteriophage efficiency and antibiotics susceptibility against Sudanese local bacteria species Escherichia coli and Staphylococcus aureus

This study was held on the in vitro tests for the bacteriophages and their efficiency comparing with the antibiotics susceptibility in destroying bacteria. In broth media the affection of the bacteriophage interactions with bacteria showed increasing of the bacteriophages and decreasing of bacteria due to culture turbidity that showed statistical significant of Escherichia coli and Staphylococcus aureus phages samples' light transmission P>0.05. On solid media the affection of the bacteriophage was recognised by the phage plaque formation on bacterial cultures. The antibiotics susceptibility against the bacteria showed statistical significant P<0.05 for E. coli and P<0.05 for S. aureus. The protein profiles of E. coli bacteriophage showed three major bands with molecular weight mass of 47, 35 and 16 kilo Dalton and 34 and 20 kDa for S. aureus phage, the band of 35 kDa was the common shared peak between the phage and the bacterial host due to the bacteriophage lytic cycle. The study showed approximately similar results for the mechanical action of the bacteriophage on the selected bacteria species and the mode of action of antibiotics.